RNA Sample Submission
Sample requirements
Quality
Only when samples meet our internal quality criteria, we can guarantee good quality data. These criteria are:
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Nanodrop OD 260/280 > 1.8
- Nanodrop OD 260/230 > 1.8
- Please pay attention that the peak of the Nanodrop profile is situated at 260 nm and not at 270 nm (this would indicate a phenol-like contamination)
- Agilent Bioanalyzer RIN (RNA Integrity Number) > 7-8
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Note that over 30 samples, you can provide your samples in a PCR plate in column orientation instead of eppendorf tubes.
Amount
For Illumina sequencing, an amount of 100 ng of total RNA is enough for the majority of the RNA applications.
Please always provide 3-5 µL extra which is to be used in our quality control steps.
Please contact us at nucleomics.lab@vib.be for more details.
Sample submission
Before sending/bringing your samples, please fill out the RNA sample submission form and upload in VIB connect.
Submission Process
Check Sample Requirements
For sequencing material using long reads, please contact us to discuss specific requirements.
Fill out the sample submission form
Please, always download the latest version instead of using a previously saved version.