DNA Sample Submission

Sample requirements

Quality

Only when samples meet our internal quality criteria, we can guarantee good quality data. These criteria are:

Nanodrop OD 260/280 > 1.8
Nanodrop OD 260/230 > 1.8
Please pay attention that the peak of the Nanodrop profile is situated at 260 nm and not at 270 nm (this would indicate a phenol-like contamination)
For high molecular weight (HMW) DNA sample preparation, please consult us as standard wetlab practices may induce DNA shearing or be detrimental to sequencing
Note that over 30 samples, you can provide your samples in a PCR plate in column orientation instead of eppendorf tubes.

Amount

For DNA applications, 500 ng of genomic DNA will usually be sufficient
Please always provide 3-5 µL extra which is to be used in our quality control steps.

Please contact us at nucleomics.lab@vib.be for more details.

Sample submission

Before sending/bringing your samples, please fill out the DNA sample submission form and upload in VIB connect.

short reads DNA Sample submission file

Amplicons or DNA samples to submit for short read sequencing

long reads amplicons sample submission file

Amplicons to submit for long read sequencing

long reads DNA PCR template sample submission file

DNA PCR template samples to submit for long read sequencing

long reads gDNA sample submission file

genomic DNA to submit for long read sequencing

PacBio custom barcoded primers

PacBio_Sequel-IIe_amplicon_barcoding_v1.xlsx (up to 2x 96 multiplexed samples per run)

PacBio_Sequel-IIe_amplicon_barcoding_v2.xlsx (up to 4x 96 multiplexed samples per run)

Sample Submission Process

Step 1

Check Sample Requirements

For sequencing material using long reads, please contact us to discuss specific requirements.

Step 2

Fill out the sample submission form

Please, always download the latest version instead of using a previously saved version.

Step 3

Upload it via VIB Connect

Step 4

Ship your samples to the VIB Nucleomics Core

/en/shipping-samples